Category:

CHROMagar™ ESBL

For overnight detection of Gram-negative bacteria producing Extended Spectrum Beta-Lactamase

Order References

Please use these references when
contacting your local distributor:

5000 mL Pack ……….. ESRT2
Included: base RT412 + supplement ES372

25 L Pack ……………. ESRT3-25
Included : base RT413-25 + supplement ES373-25

Description

Colonies Appearance

C3GR ecoli

E. coli ESBL

dark pink to reddish

C3GR klebsiella

Klebsiella, Enterobacter,

Citrobacter ESBL

metallic blue (+/- red halo)

C3GR proteus

Proteus ESBL

brown halo

Pseudomonas ESBL

translucent cream to blue

C3GR pseudomonas

Acinetobacter ESBL

cream, opaque

Performance

ESBL (Extended Spectrum β-Lactamases) are enzymes that mediate resistance to penicillins, extended-spectrum third generation cephalosporins (C3G) and monobactams. ESBL-producing Enterobacteriaceae started to appear in the 1980s, and have since emerged as some of the most significant hospital-acquired infections with Escherichia coli and Klebsiella spp. being the main actors, but other Gram-negative species have also been observed. Emergence of ESBL-producing isolates has important clinical and therapeutic implications:
 

- Resistance determinants for ESBL production are carried on plasmids that can be easily spread from organism to organism.


- The spread of resistance toward extended-spectrum cephalosporins may lead to increased prescription of more broad-spectrum and expensive drugs.


- These resistant isolates may escape detection with routine susceptibility testing performed by a clinical microbiology laboratory, which can result in adverse therapeutic outcomes.


 

Therefore, the early detection of ESBL-producing bacteria carriers is important to minimise their impact and the spread of infections and customise therapeutic patient treatment.


 

CHROMagar™ ESBL allows the detection of ESBL-producing bacteria while inhibiting the growth of other bacteria, including most of those carrying AmpC type resistance. This is an important feature because intrinsic AmpC resistance has less epidemic relevance, but often leads to ESBL false positive reading in the classical testing methods.

Intended Use :


CHROMagar™ ESBL is a selective and differential chromogenic culture medium, intended for use in the qualitative direct detection of gastrointestinal colonization with Extended-spectrum-beta-lactamase-resistant Enterobacteria (ESBL) to aid in the prevention and control of ESBL in healthcare settings. The test is performed with rectal swab and stools from patients to screen for ESBL colonization. Results can be interpreted after 18-24 h of aerobic incubation at 35-37 °C.


 

The medium can also be used as an early warning indicator for diagnostic tests of infections to signal the possible presence of multi drug-resistant bacteria. This use does not replace the institution’s protocols.


 

CHROMagar™ ESBL is not intended to diagnose infection caused by ESBL producers nor to guide nor monitor treatment for infections. A lack of growth or the absence of colonies on CHROMagar™ ESBL does not preclude the presence of ESBL-producing bacteria. Further identification, susceptibility testing, and epidemiological typing is needed on suspect colonies.

1. Fast results: Detection after overnight incubation.

4. Time and workload savings: Direct Culture from specimen. There is no need of a selective pre-enrichment.

2. Species Differentiation thanks to the chromogenic performances of supplemented CHROMagar™ Orientation. Indeed, the product is composed of a powder base CHROMagar™ Orientation and a supplement to enhance ESBL-producing bacteria.

5. Flexibility: CHROMagar™ ESBL is supplied with a more than 18 months shelf-life. This allows flexibility of use, whether in an epidemic situation with many patients to screen, or whether for random surveillance of cultures.

Composition

Technical Documents

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Scientific Publications