Food&Water Q.C.


CHROMagar™ C.perfringens

Click on the picture for
Packaging / Colonies appearance

For detection and enumeration of C. perfringens

Typical Appearance of microrganisms

Clostridium perfringens → orange
Other bacteria → blue or inhibited

Order References 

Please use these references when
contacting your local distributor:

5000 mL..........PF652

To download the certificate of analysis, please indicate your lot number below :


Medium Performance

1. To be used with pouring or surface methods (by direct streaking, spreading or filtration technique) whereas with TSC medium bacteria have to be placed between two layers of agar in order to grow in black colonies. 

2. Specific medium for Clostridium perfringens while TSC medium detects sulfate-reducing bacteria, including the non pathogens. 

3. The orange coloration makes the visualization very easy. On the other hand, the spread of the colonies' black color and the fact that they faint after a while in TSC medium (as described in the ISO 14189) makes the colony count difficult. 


Medium Description

Gain flexibility using powder rather than ready to use plates: Use the entire pack, or if there is a need for a smaller number of plates, just a portion. If kept under appropriate storage temperature, CHROMagarTM C.perfringens has more than 12 months shelf life. This flexibility is essential to avoid the waste resulting from expired-unused plates.

Please refer to our IFU and Material Safety data sheet for complete information about the medium. CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
Last Update: 27/11/2019

Focus C. perfringens

Clostridium perfringens is involved in food poisoning and animals’ infections. 

Beef, poultry, gravies, and dried or pre-cooked foods are common sources of C. perfringens infections. C. perfringens infection often occurs when foods are prepared in large quantities and kept warm for a long time before serving. 

Although C. perfringens may live normally in the human intestine, illness is caused by eating food contaminated with large numbers of C. perfringens bacteria that produce enough toxin in the intestines to cause illness.

Everyone is susceptible to food poisoning from C. perfringens. The very young and elderly are most at risk of C. perfringens infection and can experience more severe symptoms that  may last for 1 to 2 weeks. Complications, including dehydration, may occur in severe cases.” 1

CDC - Centers for Disease Control and Prevention

CHROMagarTM C.perfringens allows the detection and enumeration of Clostridium perfringens in food and water samples. Specific and selective, this medium detects the Clostridium perfringens colonies by an orange coloration, the other microorganisms being blue, metallic blue or inhibited. 

A comparative study on the use of selective media for the enumeration of Clostridium perfringens in poultry faeces


Martina Husta, Richard Ducatelle, Freddy Haesebrouck, Filip Van Immerseel, Evy Goosens Anaerobe 63, April 2020

Study of an acid phosphatase test for the confirmation of Clostridium perfringens directly from CHROMagar C.Perfringens agar medium


Laboratoire Microsept Le Lion d'Angers, France May 2020

Evaluation of media for the enumeration of C. Perfringens from Poultry Faeces


M. Hustà, E.Goossens, R. Ducatelle, F. Van Immerseel Faculty of Veterinary Medicine - Gent University NE Conference July 2018
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