Clinical Microbiology

 

CHROMagar™ Staph aureus


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Packaging / Colonies appearance
   





For isolation and direct differentiation
of Staphylococcus aureus
in clinical and industrial samples.


Typical Appearance of microrganisms

Staphylococcus aureus → pink to mauve
Other bacteria → Colourless, blue or inhibited

Order References 

Please use these references when
contacting your local distributor:

1000ml..........TA670
5000ml..........TA672
Bulk on request
To download the certificate of analysis, please indicate your lot number below :
 

 
 

Medium Performance



** Clinical Field **
  1. Easy to read: compared to Blood Agar, CHROMagar Staph aureus allows easy differentiation of mauve from blue or white colonies and is of considerable help in identifying suspect colonies.

  2. Higher sensitivity (exceeds 99%*) than Blood Agar, especially in specimens heavily loaded with gram negative flora competing with and inhibiting S.aureus. *Specificity and sensibility from scientific study: “Evaluation of CHROMagar Staph aureus, a new chromogenic medium, for isolation and presumptive identification of Staphylococcus aureus from human clinical specimens.” Gaillot O. et al. 2001. Journal of Clinical Microbiology, 38: 1587-1591. 

  3. Better selectivity and sensitivity compared to the conventional media, Mannitol Salt Agar (called Chapman Agar in Europe), which is showing an excessive rate of false positives and of false negatives. 

  4. Lower workload: Blood Agar, another conventional medium for detecting Staphylococcus aureus, requires tedious and costly examination with immunological tests of 5-10 colonies per suspected sample. With CHROMagar Staph aureus, testing a single colony with typical colour for further identification will generally be sufficient for high probability detection of Staphylococcus aureus.



 **Industrial Field **
  1. Easy to prepare: The conventional medium for S.aureus is the Baird-Parker which has to be supplemented with RPF (Rabbit Plasma Fibrinogen), rendering the plate manufacturing delicate and complex, and also reducing the shelf life of the poured plates to a couple of weeks. On the contrary, CHROMagar Staph aureus comes with all the compounds already in the agar (no need of any supplement).

  2. Fast: The results on Baird Parker have to be read after 48h of incubation while with CHROMagar Staph aureus the results are available after only 24 h.




    Medium Description


 

    Gain flexibility using powder rather than ready to use plates: Use the entire pack, or just a portion if there is a need for a smaller number of plates. If kept under appropriate storage temperature, CHROMagar Staph aureus has a 2 years shelf of life. This flexibility is essential to avoid the waste resulting from expired-unused plates. 



Please refer to our IFU and Material Safety data sheet for complete information about the medium.
CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.

All pictures of our products are CHROMagar property and should not be used without our acceptance.      Last Update: 26-Oct-2017

Focus on Staph aureus


Staphylococcus aureus is a major pathogenic bacterium found in clinical specimens and in food industry products. Staph. aureus is a commensal human germ (50% of the population hosting S.aureus) but can be an opportunistic agent due to its pyogenic and toxinogenic behaviour. It represents a major public health problem.



Staph aureus Epidemiologic Issues



**Clinical Field **
S. aureus has long been recognized as one of the most important bacteria that cause disease in humans. It is the leading cause of skin and soft tissue infections. Although most Staph infections are not serious, S. aureus can cause serious infections such as bloodstream infections, pneumonia, or bone and joint infections.
Anyone can develop a Staph aureus infection, although certain groups of people are at greater risk: newborn infants, breastfeeding women, people with chronic conditions, those with skin injuries or disorders, intravenous catheters, surgical incisions, and those with a weakened immune system.
Another major concern is related to the increasing number of nosocomial infection cases due to Staphylococcus aureus.



**Industrial Field **
Human beings are the main reservoir of S. aureus. A carrier contaminates the surrounding environment when coughing, sneezing and by touching food with a hand having a staphylococcus-infected lesion. It is often found in the environment and on food preparation surfaces and also in certain uncooked foods (dairy products, salads, sandwiches,..)
It is important to check the presence of S. aureus before and after the foodstuff sterilisation process. Even if S. aureus is destroyed by heat, its presence in raw materials remains dangerous. Indeed, enterotoxins produced by S. aureus are heat-resistant and can contaminate well-cooked food. According to international QC guide lines, if the level of S. aureus is over a certain limit (ex: meat, usually over 103cfu/gr), improvements in hygiene have to be made in the production facilities as well as in raw material selection.
 

Optimal detection of Staphylococcus aureus from clinical specimens using a new chromogenic medium.

2004

Samra Z., Ofir O., Bahar J.
2004. Diagnostic Microbiology and Infectious Disease,
49 : 243-247.
PUBLICATION

Use of a new chromogenic culture medium facilitates detection of Staphylococcus aureus in a screening program.

2003

Frei R. et al.
2003. Abstract presented at the Annual Meeting Swiss Society for Microbiology in Basel (Suisse).
PUBLICATION

Performance of the chromogenic medium CHROMagar Staph aureus and the Staphylochrom coagulase test in the detection and identification of Staphylococcus aureus in clinical specimens.

2001

Carricajo A., Freydière A.M. et al.
2001, Journal of Clinical Microbiology, 39 : 2581-2583.
PUBLICATION
 
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