Food&Water Q.C.

 

CHROMagar™ Vibrio


Click on the picture for
Packaging / Colonies appearance
   





For isolation and detection
of
V.parahaemolyticus, V.vulnificus
and
V.cholerae.

Typical Appearance of microrganisms

V.parahaemolyticus → mauve
V.vulnificus / V.cholerae
→ green blue to turquoise blue
V.alginolyticus → colourless

Order References 

Please use these references when
contacting your local distributor:

1000ml...........VB910
5000ml...........VB912
25L.................VB913-25
Bulk on request
To download the certificate of analysis, please indicate your lot number below :
 
 
 

Medium Performance


  1. 3 different clear and intense colony colourations thanks to its powerful chromogenic technology. Easy reading especially when compared to the conventional TCBS medium based on sucrose fermentation revealed with a pH indicator.

  2. V. alginolyticus remains colourless in CHROMagar Vibrio, avoiding any interference with the detection of other species.

  3. Clear differentiation between V. parahaemolyticus and V. vulnificus, both sucrose (-) on TCBS.

  4. Unrivalled medium in the chromogenic media field.

  5. Recovery of Vibrio is greater than with TCBS agar, even if using an enrichment broth.

  6. Fewer false negatives than with TCBS agar.


Media Description





Gain flexibility using powder rather than ready to use plates: Use the entire pack, or just a portion if there is a need for a smaller number of plates. If kept under appropriate storage temperature, CHROMagar Vibrio has 3 years shelf life. This flexibility is essential to avoid the waste resulting from expired-unused plates.

-Suitable with ISO/TS 21872:2007 : Microbiology of food and animal feeding stuffs -- Horizontal method for the detection of potentially enteropathogenic Vibrio spp. -- Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae ISO/TS 21872-1:2007 specifies a horizontal method for the detection of the two main pathogenic Vibrio species causing intestinal illness in humans: V. parahaemolyticus and V. cholerae. Part 2: Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
-Referenced in the Chinese standard for microbiological examination of food hygiene GB/T 4789.7-2008.  

Please refer to our IFU and Material Safety data sheet for complete information about the medium. CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
Last Update: 19-Jun-2012

Focus on Vibrio Species


Naturally present on marine plants and animals, Vibrio genus counts over 20 species among which four represent a serious public health hazard.


    Vibrio Epidemiologic Issues


  • V.cholerae, serogroups O1 and O139, often cause cholera through water and food contamination. Emerging cyclically, cholera is considered to be endemic in many countries as a virulent disease causing severe diarrhea and dehydration. The number of cholera cases reported to the W.H.O. in 2006 rose dramatically, reaching the level of the 1990s. Around 240,000 cases were reported from 52 countries, including about 6300 deaths.

  • V. parahaemolyticus and V. vulnificus are largely involved in foodborne diseases from seafood, causing septicaemia, wound infections, and gastroenteritis. CDC reports an estimated 47% increase of Vibrio infections in the US (1996-1998 to 2008), about 8000 illnesses yearly. Vibrio infections are also commonly reported in areas of Asia and Oceania, linked to the high consumption of seafood. Despite the fact that V. parahaemolyticus is the most commonly reported species causing infection, V. vunificus has become increasingly prevalent and is now associated with 94% of reported deaths.

  • V. alginolyticus is less common but is a major pathogen issue for oyster producers since it can lead to major production losses. If detected, it can prevent contamination of other oyster production sites.
 

Characterization of Vibrio spp. on CPC+, CHROMagar Vibrio, and TCBS, and Proposed Cross-Plating Method for Isolation of Vibrio vulnificus from Environmental Samples

2012

Tiffany Williams*, Brett Froelich, and James D. Oliver Department of Biology, University of North Carolina at Charlotte POSTER ASM 2012 P2552
PUBLICATION

Comparison of two selective and differential media for the isolation of Vibrio vulnificus from the environment

2011

T.C. Williams et al. University of North Carolina at Charlotte, 9201 University City Blvd, Charlotte, NC 28223
PUBLICATION

Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus

2010

Angela Di Pinto, a, , Valentina Terioa, Lucia Novelloa and Giuseppina Tantillo a Dipartimento di Sanitá Pubblica e Zootecnia, Sezione di Controllo e Sicurezza degli Alimenti, Facoltá di Medicina Veterinaria, Universitá degli Studi di Bari Aldo Moro, Strada Prov. le per Casamassima, km 3, 70010 Valenzano, Bari, IT
www.sciencedirect.com/science/article/pii/S0956713510001957
 
more publications