Medium Performance
- Fast: 24h incubation.
- A membrane filtration method can be used for detection from 100 ml of water, the inoculated membrane is placed, sample uppermost, on the agar plate.
- Simple to prepare: with CHROMagar Pseudomonas the pre-weighed agar powder is mixed with the required volume of distilled water.
- Easy to read: one unique intensified colour for Pseudomonas.
- Simple to use: colonies can be viewed under normal lighting conditions. Pseudomonas colonies develop with an intense blue-green colony colour, clearly visible to the naked eye.
Medium Description
Gain flexibility using powder rather than ready to use plates: Use the entire pack, or just a portion if there is a need for a smaller number of plates. If kept under appropriate storage temperature, CHROMagar Pseudomonas has a 5 years shelf of life. This flexibility is essential to avoid the waste resulting from expired-unused plates.
Please refer to our IFU and Material Safety data sheet for complete information about the medium.
CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
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Last Update: 19-Jun-2012
Focus on Pseudomonas
Pseudomonas are ubiquitous bacteria found in the soil, on plants and in freshwater and marine habitats. Many strains can grow at low temperature (psychrophilic strains) and may contaminate food or pharmaceutical products stored in the refrigerator.
Pseudomonas strains can occasionally be isolated from the intestinal flora of humans or animals.
Pseudomonas Epidemiologic Issues
Clinical issues:
Their ability to resist to many antibiotics and antiseptics explains
their increasingly frequent presence in hospitals. They behave as
opportunistic pathogens, often causing nosocomial infections. According
to data from the CDC’s National Nosocomial Infections Surveillance
System, P. aeruginosa can be rated as the Number 1 cause of intensive care unit (ICU)–related pneumonia.
Drinking water in hospitals may also be a source of serious infection
for patients with a compromised immune system or for patients in burn
care units where it prevents the regeneration of healthy tissue.
Pseudomonas strains have also been shown to be harmful to sufferers of cystic fibrosis.
Pseudomonas aeruginosa is among the bacteria most frequently isolated from drinking water in health facilities.
Food industry and environmental issues:
P. aeruginosa is a valid
indicator allowing verification of recreational water disinfection
efficacy. This parameter is currently used as a criterion in the
regulation of wading and swimming pools. Moreover, the absence of P. aeruginosais important not only in terms of its role as an indicator, but also
because it is an opportunistic pathogen whose transmission is often
associated with water.
Other forms of Pseudomonas bacteria are known to cause food spoilage at low temperatures. These psychrophillic Pseudomonas strains include: P. fragi, which causes spoilage of dairy products, P. taetrolens which causes mustiness in eggs and P. mudicolens and P. lundensis, which cause spoilage of milk, cheese, meat, and fish, but are rarely a cause of food poisoning.
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