Food&Water Q.C.

 

CHROMagar™ ECC


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Packaging / Colonies appearance
  





For the simultaneous detection and enumeration of E.coli and other coliforms
in food or water samples.
 


Typical Appearance of microrganisms

E.coli → Blue
Other Coliforms → Mauve
Other bacteria → Colourless or inhibited.

Order References 

Please use these references when
contacting your local distributor:

1000 mL ............EF320
5000 mL ............EF322
25 L...................EF323-25
Bulk size......on request
To download the certificate of analysis, please indicate your lot number below :
 

 
 

Medium Performance


  1. CHROMagar ECC allows simultaneous detection and differentiation between E.coli and coliforms in one medium!
    This is helpful to determine if there is organic contamination (coliforms) or faecal contamination (E.coli). The use of this technique involves less work in comparison with traditional methods (MI Agar).

  2. Easy to read thanks to the high colour contrast between colonies. There is no mixing of both colours (unlike some other chromogenic media on the market). Colonies are either red or blue (no metallic blue nor purple). 

  3. The media can be used as a poured plate, for isolation and as a support for membrane filtration techniques.



Media Description



Gain flexibility using powder rather than ready to use plates: Use the entire pack, or, just a portion if there is a need for a smaller number of plates. If kept under appropriate storage temperature, CHROMagar ECC has a 3 years long shelf of life. This flexibility is essential to avoid the waste resulting from expired-unused plates. In case of high quantity of Pseudomonas or Aeromonas, it is possible to add Cefsulodine. 

Listed in BAM.

Please refer to our IFU and Material Safety data sheet for complete information about the medium.
CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.

All pictures of our products are CHROMagar property and should not be used without our acceptance.
Last update: 19-Jun-2012

Focus on E.coli and Coliforms


Different regulations exist for E.coli/Coliform research in water and food samples. This can be explained by the importance of these germs in determining water and food safety.
Worldwide, water and food quality control for human consumption are based on detecting the presence/absence of E.coli and coliforms.
Coliforms, Enterobacteriacae able to ferment lactose (lactose positive Enterobacteriacae), are bacteria present in human and warm blooded animals intestinal flora, in the soil and water. Coliforms are proof of organic, environmental or faecal contamination. Faecal contamination, due to colifoms coming from animal waste, consists mainly of Escherichia coli and thermotolerant Klebsiella.


E.coli and Coliforms Epidemiologic Issues


E.coli can contaminate drinking water when the water treatment system is inadequate or during periods of very high rainfalls.
Monitoring of food and water production is essential. High contamination may lead to suspension of the water supply and food recall by supermarkets.

In the U.S.A. the EPA recommendations through the Total Coliform Rule (TRC) are:
- <100 CFU/100ml for a body-contact recreation water quality.
- <1000 CFU/100ml for a fishing and boating water quality.
- <1 CFU/100ml for a drinking water quality.

 

COMPARISON OF PETRIFILM AND CHROMAGAR ECC FOR ISOLATION OF E. COLI FROM CHICKEN

2005

Submitted to: International Association for Food Protection Publication Date: July 15, 2005 Citation: Bailey, J.S., Cray, P.J., Berrang, M.E., Plumblee, J. 2005. Comparison of petrifilm and chromagar ecc for isolation of e. coli from chicken [abstract].International Association for Food Protection. 171:T4-08.
PUBLICATION

Trial report : “Comparison between CHROMagar ECC method for the detection and enumeration of Escherichia coli and coliforms and FIL-IDF N°73B: 1998 method for the enumeration of coliforms

2001

2001, National Institute of Industrial Technology
PUBLICATION

Comparison and recovery of Escherichia coli and thermotolerant coliforms in water with a chromogenic medium incubated at 41 and 44.5°C.

1999

Alonso J. L. et al. .
1999. Applied and Environmental Microbiology, 65 : 3746-3749.
PUBLICATION
 
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