Medium Performance
1. To be used with pouring or surface methods (by direct streaking, spreading or filtration technique) whereas with TSC medium bacteria have to be placed between two layers of agar in order to grow in black colonies.
2. Specific medium for Clostridium perfringens while TSC medium detects sulfate-reducing bacteria, including the non pathogens.
3. The orange coloration makes the visualization very easy. On the other hand, the spread of the colonies' black color and the fact that they faint after a while in TSC medium (as described in the ISO 14189) makes the colony count difficult.
Medium Description
Gain flexibility using powder rather than ready to use plates: Use the entire pack, or if there is a need for a smaller number of plates, just a portion. If kept under appropriate storage temperature, CHROMagarTM C.perfringens has more than 12 months shelf life. This flexibility is essential to avoid the waste resulting from expired-unused plates.
Please refer to our IFU and Material Safety data sheet for complete information about the medium. CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
Last Update: 15/07/2019
Focus C. perfringens
“Clostridium perfringens is involved in food poisoning and animals’ infections.
Beef, poultry, gravies, and dried or pre-cooked foods are common sources of C. perfringens infections. C. perfringens infection often occurs when foods are prepared in large quantities and kept warm for a long time before serving.
Although C. perfringens may live normally in the human intestine, illness is caused by eating food contaminated with large numbers of C. perfringens bacteria that produce enough toxin in the intestines to cause illness.
Everyone is susceptible to food poisoning from C. perfringens. The very young and elderly are most at risk of C. perfringens infection and can experience more severe symptoms that may last for 1 to 2 weeks. Complications, including dehydration, may occur in severe cases.” 1
1 CDC - Centers for Disease Control and Prevention
CHROMagarTM C.perfringens allows the detection and enumeration of Clostridium perfringens in food and water samples. Specific and selective, this medium detects the Clostridium perfringens colonies by an orange coloration, the other microorganisms being blue, metallic blue or inhibited.
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