Food&Water Q.C.


CHROMagar™ E.coli

Click on the picture for
Packaging / Colonies appearance

For detection and enumeration of E. coli
in food, water and environmental samples.

Typical Appearance of microrganisms

E. coli → blue
Other gram negative bacteria → colourless
Gram positive → inhibited

Order References 

Please use these references when
contacting your local distributor:

5000 mL..........EC168
25 L...............EC169-25

To download the certificate of analysis, please indicate your lot number below :


Medium Performance

  1. 24 h detection. 

  2. Easy reading and interpretation: The general food and water standards limits are usually zero to single figure E. coli cfu per gram and thus it is important to detect and enumerate them accurately. With CHROMagarTM E.coli, colonies of E. coli develop with an intense blue colour - thus making detection and enumeration of this important hygiene indicator as simple as possible.

  3. Lighter Workload: Traditional E. coli detection methods are extremely tedious and labor-intensive, requiring experimental studies of many colonies.

  4. Quality: CHROMagarTM media contain 5 % more agar than other media on the market. This helps considerably with the application and streaking of the sample onto the plate.

Medium Description

Gain flexibility using powder rather than ready to use plates: Use the entire pack, or just a portion if there is a need for a smaller number of plates. If kept under appropriate storage temperature, CHROMagarTM E.coli has a shelf life of more than 18 months. This flexibility is essential to avoid the waste resulting from expired-unused plates. For detailed preparation procedure, please refer to our IFU.

-Recommended by USDA SOP No.: MDP-QA-03 (Page 1 of 6 Title: Quality Assurance (QA) Controls Revision: 06 Replaces: 04/01/09 Effective: 09/25/09, United States Department of Agriculture,Agricultural Marketing Service, Science & Technology, Microbiological Data Program) for QA Control Strains.
-Listed in BAM

Please refer to our IFU and Material Safety data sheet for complete information about the medium.
CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.

All pictures of our products are CHROMagarTM property and should not be used without our acceptance.
                                                                                                     Last Update: 20-May-2019

Focus on E. coli

Coliforms are viewed as indicators of contamination by organic, environmental or faecal material.
Contamination by faecal material from animals can be shown by the detection of Escherichia coli in the sample. E. coli can contaminate drinking water when the water treatment system is inadequate or during periods of very high rainfalls.
Monitoring of food and water production is essential. High contamination may lead to the suspension of the water supply and food recall by supermarkets.

Regulations concerning bathing water:
→ European directive from 1976: 2.000 Escherichia coli (E. coli) bacteria for 100 mL of water.
→ New directive in 2006 (effective in France in 2015): 500 E. coli per 100 mL.

E. coli Epidemiologic Issues

The presence of E. coli indicates faecal contamination and potential risk of dangerous pathogens such as bacteria like Vibrio cholerae, Salmonella, Pseudomonas etc…, or viruses and intestinal parasites.
The infections resulting from ingestion of contaminated matter can be dangerous and life-threatening.


Quantitative determination of Escherichia coli in water using CHROMagar E.coli.


Alonso J. L. et al.
1996. Journal of Microbiological Methods,
25 : 309-315.

Dénombrement de Escherichia coli au moyen de géloses chromogènes sélectives - comparaison avec la méthode officielle.


Grand M. et al.
1996. Trav. Chim. Aliment. Hyg.,
87 : 623-630.

Comparison of enumeration of E.coli on CHROMagar E.coli and MPN methods.


Study by Weissman S., Israël.
more publications