Clinical Microbiology


CHROMagar™ E.sakazakii

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Packaging / Colonies appearance

For detection of E. sakazakii (Cronobacter spp) 
according to ISO/TS 22964 standard 

Typical Appearance of microrganisms

E. sakazakii  green to blue 
Other Gram (-)  inhibited, colourless or light purple/translucent colonies 
Gram (+) bacteria  inhibited

Order References 

Please use these references when
contacting your local distributor:
5000 mL .......... CS812
This product code is composed of a powder base CS812
To download the certificate of analysis, please indicate your lot number below :


Medium Performance

ISO/TS 22964 STANDARD: CHROMagarTM E.sakazakii is a chromogenic medium for detection of E. sakazakii in food, mainly powdered milk, according to the ISO/TS 22964 standard. 

INTENSE BLUE COLONIES: E. sakazakii will grow in blue colonies while most other bacteria will be inhibited or grow in colourless colonies, after Incubation at 44 °C for 24 h.

Medium Description  

CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach. All pictures of our products are CHROMagar™ property and should not be used without our acceptance. 
                                                            Last Update : 30-Nov-2018

Focus on CHROMagar™ E.sakazakii

« Enterobacter sakazakii is a gram-negative, non-spore-forming bacterium belonging to the Enterobacteriaceae family <……>.
 It has been implicated in outbreaks causing meningitis or enteritis, especially in infants. In the few outbreaks reported 20 % to >50 % of the infants who contracted the disease died. For survivors, severe lasting complications can result including neurological disorders. The outcome related to adult disease seems to be significantly milder.” « The infant food industry should be encouraged to reduce the concentration and prevalence of E. sakazakii in both the manufacturing environment and powdered infant formula. To this end, the infant food industry should consider implementing an effective environmental monitoring programme and the use of Enterobacteriaceae rather than coliform testing as an indicator of hygienic control in factory production lines. » Extracts from WHO Microbial Risk Assesment Feb-2007.

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