Clinical Microbiology

 

CHROMagar™ STEC


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Packaging / Colonies appearance
  





For detection of 
Shiga-Toxin producing E.coli (STEC)

Most common Shiga-Toxin E.coli serotypes 
→ mauve
Other Enterobacteriacae 
→ colourless, blue or inhibited
Gram positive bacteria 
→ inhibited

Using the optional CHROMagar STEC-O104 supplement:
STEC O104:H4 → mauve
Other → colourless, blue or inhibited

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Order References 

Please use these references when
contacting your local distributor:

CHROMagar STEC:
1000 ml pack .............................................. ST160
Product = powder base ST160(B) + supplement ST160(S)
5000 ml pack .............................................. ST162
Product = powder base ST162(B) + supplement ST162(S)



To download the certificate of analysis, please indicate your lot number below :
 
 
 

Medium Performance

  • Easy reading: a majority of STEC strains grow in mauve colony color, while other bacteria grow in blue, colourless or are inhibited. 
  • Highly STEC selective medium: excellent tool for large number of samples screening procedures. 
  • Worldwide premiere: unique medium in the market for STEC detection. 
  • Flexibility: it can be supplemented with additional compounds to render it even more selective for the strain causing an outbreak.



    • Medium Description


     

CE Marked Product

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    last update: 28-Mar-13

Focus on STEC E.coli

An increasing and worrisome number of studies have lately shown that, non-O157 ShigaToxin-producing E.coli (STEC) have been responsible for foodborne poisoning outbreaks. The CDC has also reported warnings about this potential risk: 

“ Disease caused by Shiga toxin-producing Escherichia coli (STEC) ranges from self-limiting diarrhea to hemorrhagic colitis and hemolytic uremic syndrome (HUS). Serotype O157:H7, the most frequently implicated STEC causing hemorrhagic colitis and HUS, has been isolated from large foodborne outbreaks, as well as sporadic cases, in North America and abroad. However, 60 STEC serotypes have been implicated in diarrheal disease, and several non-O157:H7 serotypes have been implicated as the cause of foodborne outbreaks and HUS in the United States, Europe, and Australia. Studies from Canada, Europe, Argentina, and Australia suggest that non- O157:H7 STEC infections are as prevalent, or more so, than O157:H7 infection.” 

CDC report « Prevalence of Non-O157:H7 Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Samples [...]» 

Therefore, several regulatory authorities urge worldwide food industry to implement measures to control the absence of such organisms in their production. 
In many cases, laboratories have limited their search for pathogenic E.coli to the common O157 serotype. This is due, among other reasons, to the fact that there were no available selective culture media for non-O157 E.coli. 
CHROMagar STEC is designed to fill this gap: detection, as mauve colonies, of not only the classical STEC O157, but also many other serotypes.






 

Usability and Performance of CHROMagar STEC Medium in Detection of Shiga Toxin-Producing Escherichia coli Strains

2013

J. Clin. Microbiol. 2012, 50(11):3586. Jari J. Hirvonen, Anja Siitonen and Suvi-Sirkku Kaukoranta
PUBLICATION

Improving diagnostics of diarrheagenic Escherichia coli by use of a new chromogenic medium

2012

Susanne I. et al - Germany Institute of Medical Microbiology and Hygiene, University of Luebeck Institute of Medical Microbiology and Infection Epidemiology, University of Leipzig Poster ECCMID London 2012
PUBLICATION

A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables

2011

Markus Tzschoppe, Annett Martin, Lothar Beutin International Journal of Food Microbiology 2011
PUBLICATION
 
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