Clinical Microbiology

 

CHROMagar™ KPC


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Packaging / Colonies appearance
  





For Detection of gram-negative bacteria
with a reduced susceptibility
to most of the carbapenem agents.


Typical Appearance of microorganisms

E.coli CarbapenemR → Dark pink to reddish
Klebsiella, Enterobacter, Citrobacter CarbapenemR
→ Metallic blue
Pseudomonas CarbapenemR → Cream, translucent
Other bacteria → Usually inhibited

Order References 

Please use these references when
contacting your local distributor:
 
Product = base powder CHROMagar Orientation + CHROMagar KPC supplement

5000ml.............. KPRT2
(base powder ref:RT412 + supplement ref:KP102)
25L.............. KPRT3-25
(base powder ref:RT413-25 + supplement ref:KP103-25)
To download the certificate of analysis, please indicate your lot number below :
 

 
 

Medium Performance

  1. Detection of gram-negative bacteria expressing a reduced susceptibility to antibiotics of the carbapenem family.

  2. Detection of NDM-1: this new resistance gene recently reported, has the ability of destroying carbapenems. Bacteria harbouring this resistance mechanism (a metallo-betalactmase) can easily be screened with CHROMagar KPC.

  3. Species identification thanks to the CHROMagar™ Orientation base.

  4. Time and workload savings: there is no need for a selective pre-enrichment medium. Direct plating of the sample is possible.

  5. Requires only 18-24 hours of incubation.

  6. Unique commercially available culture medium for the detection of bacteria having reduced suceptibility to carbapenems.

  7. Flexibility: CHROMagar KPC Supplement is supplied with a shelf-life of about 2 years. This allows for flexibility of use, whether in an epidemic situation with many patients to screen, or whether for random surveillance of cultures.


Media Description




    Gain flexibility using powder rather than ready to use plates: Use the entire pack, or if there is a need for a smaller number of plates, just a portion. If kept under appropriate storage temperature, CHROMagar Orientation and CHROMagar KPC Supplement have a 2 years shelf life. This flexibility is essential to avoid the waste resulting from expired-unused plates.

In the USA: For Research Use Only

Please refer to our notice and Material Safety data sheet for complete information about the medium.
CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
             Last Update: 26-Oct-2017

Focus on KPC Resistance

KPC carbapenemase is an enzyme class, first found in Klebsiella pneumoniae isolates (hence, the name).
However, it can be produced by other organisms including Serratia spp., Enterobacter spp., E. coli, Citrobacter freundii and Salmonella enterica.


KPC resistance Epidemiologic Issues


Carbapenems are the last resort in treating many serious gram-negative infections. However, production of these enzymes results in resistance to penicillins, cephalosporins (i.e., cefepime, ceftriaxone), carbapenems (i.e., meropenem, ertapenem), and aztreonam, thereby making these pathogens truly multidrug-resistant and making their treatment very challenging.

« KPC-producing bacteria have demonstrated a remarkable ability to disseminate with interfacility, interstate, and international transmission having been documented. » CDC 2008-R-24.
Thus, in order to limit the spread of these serious pathogens, rapid detection, followed by implementation of adequate infection control methods, is essential.
 

Laboratory evaluation of different agar media for isolation of carbapenem-resistant Acinetobacter spp.

2014

J.Moran-Gilad; A.Adler; D.Schwartz; S.Navon-Venezia; Y.Carmeli Eur J Clin Microbiol Infect Dis
PUBLICATION

Evaluation of three selective chromogenic media, CHROMagar ESBL, CHROMagar CTX-M and CHROMagar KPC, for the detection of Klebsiella pneumoniae producing OXA-48 carbapenemase

2013

Michael Hornsey, Lynette Phee, Neil Woodford, Jane Turton, Daniele Meunier,Claire Thomas, David W Wareham
PUBLICATION

Laboratory Response to a KPC Outbreak at the NIH Clinical Center

2012

Anna Lau1*, Stella Antonara1, Frida Stock1, Tara Palmore2, Adrian Zelazny1 1Microbiology Service, Department of Laboratory Medicine;2Hospital Epidemiology Service, Clinical Center, Na@onal Ins@tutes of Health, Bethesda, MD, US Control number: #3618
PUBLICATION
 
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