Clinical Microbiology

 

LIM RambaQUICK™ StrepB Method


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For Rapid detection of Streptococcus agalactiae from swabs

Order References 

Please use these references when
contacting your local distributor:
 
LIM RambaQUICKTM Strep B Enrichment broth powder in 5000ml pack: LB082 


LIM RambaQUICKTM StrepB Method to prepare 5000 ml of broth (1000 tests): 1 pack of LB082 + 4 packs of SB282

To download the certificate of analysis, please indicate your lot number below :
 

 
 

Medium Performance

For Rapid detection of Streptococcus agalactiae from swabs

INHIBITION OF MOST ENTEROCOCCI
LIM RambaQUICKTM StrepB is a selective enrichment broth designed to inhibit Enterococci, while allowing growth of GBS, and, therefore to raise the sensitivity of the prepartum screening method.

BROTH INOCULATION
Classical Method recommends overnight broth incubation for classical LIM. LIM RambaQUICKTM StrepB can be used also with overnight incubation, but has an optimal differentiation at 6h only. 

EASY INTERPRETATION OF THE SUBCULTURE PLATE
Easier reading of CHROMagarTM StrepB plate thanks to an intense mauve colony colouration.
 

Medium Description




CHROMagar™, RambaQUICK™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
All pictures of our products are CHROMagar property and should not be used without our acceptance.
                                                                                          Last update: 26-Oct-2017

Focus on StrepB

 The centers for Disease Control and Prevention guidelines for the prevention of perinatal Group B Streptococcal (GBS) infections recommend that pregnant women be tested at 35 to 37 weeks of pregnancy or GBS carriage by selective enrichment broth of vaginal/anorectal swab samples, followed by subculture on blood agar or chromogenic plates. 

Enterococci growth properties are very similar to the GBS. Enterococci are able to hinder the growth of GBS in traditional broths by competition. Thus, a source of false negative results still remains since the traditional broth enrichments (Todd-Hewitt, LIM) are not dedicated to the inhibition of Enterococci, which are actually part of the anorectal flora.

Despite the fact that CHROMagarTM StrepB helps differentiate Enterococci from GBS by producing colonies of a distinct colour the risk remains of high levels of Enterococci growth may obscure GBS colonies.

RambaQUICKTM StrepB is a selective enrichment additive designed to reduce the growth or Enterococci, while allowing growth of GBS. 

 



 

Comparison of culture-based methods for Group B Streptococcus detection in screening samples from pregnant women

2014

B.Berg, M.Hatto, W.LeBar, D.Newton, C.Young University of Michigan Health System, Ann Arbor, MI
PUBLICATION

Prepartum vaginal/anorectal Group B Streptococcus screening: Improvement of the enrichment step by the broth additive RambaQUICK StrepB

2012

Didier-Marc Poisson, Marie-Liesse Evrard. Microbiology Laboratory & Gynaecology Obstetric Ward, Centre Hospitalier Régional Orléans, FR
PUBLICATION
 
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