Clinical Microbiology

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LIM RambaQUICK™ StrepB Method

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Packaging / Colonies appearance

For Group B Streptococcus (GBS) screening

Order References

Please use these references when
contacting your local distributor:

CHROMagarTM Strep B ............. 5000 mL ....... SB282 (250 plates)

LIM RambaQUICK^TM Strep B ..... 5000 mL ....... LB082 (1000 tests)

Product Leaflet
Instruction For Use Instruction for Use for US Customers
(Under FDA Regulatory Guidance)
Material Safety Data Sheet

Medium Performance

For Group B Streptococcus (GBS) screening

INHIBITION OF MOST ENTEROCOCCI

LIM RambaQUICK^TM StrepB is a selective enrichment broth designed to inhibit Enterococci, while allowing growth of GBS, and, therefore to raise the sensitivity of the prepartum screening method.

BROTH INOCULATION

Classical Method recommends overnight broth incubation for classical LIM. LIM RambaQUICK^TM StrepB can be used also with overnight incubation, but has an optimal differentiation at 6h only.

EASY INTERPRETATION OF THE SUBCULTURE PLATE

Easier reading of CHROMagar^TM StrepB plate thanks to an intense mauve colony colouration.

Medium Description

CHROMagar™, RambaQUICK™, AquaCHROM™ are trademarks created by Dr. A. Rambach.

All pictures of our products are CHROMagar™ property and should not be used without our acceptance.

Last update: 21-Jan-2020

Focus on Group B Streptococcus (GBS)

The centers for Disease Control and Prevention guidelines for the prevention of perinatal Group B Streptococcal (GBS) infections recommend that pregnant women be tested at 35 to 37 weeks of pregnancy or GBS carriage by selective enrichment broth of vaginal/anorectal swab samples, followed by subculture on blood agar or chromogenic plates.

Enterococci growth properties are very similar to the GBS. Enterococci are able to hinder the growth of GBS in traditional broths by competition. Thus, a source of false negative results still remains since the traditional broth enrichments (Todd-Hewitt, LIM) are not dedicated to the inhibition of Enterococci, which are actually part of the anorectal flora.

Despite the fact that CHROMagar^TM StrepB helps differentiate Enterococci from GBS by producing colonies of a distinct colour the risk remains of high levels of Enterococci growth may obscure GBS colonies.

RambaQUICK^TM StrepB is a selective enrichment additive designed to reduce the growth or Enterococci, while allowing growth of GBS.

Comparison of culture-based methods for Group B Streptococcus detection in screening samples from pregnant women

2014

B.Berg, M.Hatto, W.LeBar, D.Newton, C.Young University of Michigan Health System, Ann Arbor, MI

PUBLICATION

Prepartum vaginal/anorectal Group B Streptococcus screening: Improvement of the enrichment step by the broth additive RambaQUICK StrepB

2012

Didier-Marc Poisson, Marie-Liesse Evrard. Microbiology Laboratory & Gynaecology Obstetric Ward, Centre Hospitalier Régional Orléans, FR

PUBLICATION

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