Clinical Microbiology


CHROMagar™ Orientation

Click on the picture for
Packaging / Colonies appearance

For isolation and differentiation
of urinary tract pathogens.

Typical Appearance of microrganisms

E.coli → dark pink to reddish
Enterococcus → turquoise blue
Klebsiella, Enterobacter, Citrobacter → metallic blue
Proteus → brown halo
Pseudomonas → cream, translucent
S.aureus → golden, opaque, small
S.saprophyticus → pink, opaque, small

Order References 

Please use these references when
contacting your local distributor:

5000ml........... RT412
25L................ RT413-25
Bulk .............. on request
To download the certificate of analysis, please indicate your lot number below :


Medium Performance

  1. Isolation of a variety of microorganisms: the major target of this medium is the detection of urinary tract pathogens but CHROMagarTM Orientation has a broader application as a general nutrient agar for the isolation of various microorganisms. CHROMagarTM Orientation can also be used to differentiate various micro-organisms in other infected areas; e.g. scars. In addition, CHROMagarTM Orientation is useful when supplemented with various antibiotics in detecting increasingly important nosocomial and multidrug resistant microorganisms (See CHROMagar ESBL, CHROMagar KPC supplements). 

  2. It presents an instant palette of colours to obtain a large spectrum of differentiation of species. CHROMagar Orientation has several advantages over traditional media:
    - allows in most cases full differentiation of the pathogens 
    - allows for reliable detection, enumeration and presumptive identification of urinary tract pathogens 
    - easier recognition of mixed growth 
    - provides higher detection rates

  3. High detection of minor populations: the proper use of CHROMagar Orientation will correctly pinpoint the presence of a minor population and will help to establish the right diagnosis and therapy.

  4. Save time and reduce workload thanks to a high specificity: The most common UTI pathogen is E.coli, found in 40-70% of infections. CHROMagar Orientation has a specificity of 99,3%* for E.coli, rendering the species confirmatory test largely unnecessary. One plate of CHROMagarTM Orientation will give the same information as the combination of the 3 classical plates used for UTI analysis (blood agar, CLED and Mac Conkeyagar). Moreover since it is easy to differentiate mixed flora on CHROMagarTM Orientation, antimicrobial susceptibility tests can be performed directly from primary isolates without the need of subcultures.
    * Merlino, J. et al. 1996. Evaluation of CHROMagar Orientation for Differentiation and Presumptive Identification of Gram-Negative Bacilli and Enterococcus Species, J.C.M. 34: 1788-1793.

Medium Description

Gain flexibility using powder rather than ready to use plates: Use the entire pack, or if there is a need for a smaller number of plates, just a portion. If kept under appropriate storage temperature, CHROMagarTM Orientation has a 2 years shelf life. This flexibility is essential to avoid the waste resulting from expired-unused plates.

Please refer to our notice and Material Safety data sheet for complete information about the medium.
CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
Last Update: 26-Oct-2017

Focus on Urinary tract pathogens

Urinanalysis is the most common clinical microbial test.
For instance, in France in 2007, out of 10 million bacteriology tests carried out, 6 million (60%) were urinanalyses.

Any workload reduction related to this analysis will dramatically improve the efficiency of the laboratory.

Evaluation of use of a new chromogenic agar in detection of urinary tract pathogens.


Samra Z. et al.
1998. Journal of Clinical Microbiology, 36: 990-994.

Evaluation of a new medium for isolation, differentiation and presumptive identification of microorganisms in urinary tract infections.


Samra Z. et al.
1997. Poster presented at ASM’97 in Miami (USA).

Evaluation of CHROMagar Orientation for differentiation and presumptive identification of Gram negative bacilli and Enterococcus species.


Merlino J. et al.
1996 Journal of Clinical Microbiology, 34: 1788-1793.
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