Clinical Microbiology



Click on the picture for
Packaging / Colonies appearance

For isolation and differentiation
of Colistin resistant Gram-negative pathogens

Typical Appearance of microrganisms

COL-R E.coli → dark pink to reddish
COL-R Coliforms → metallic blue
COL-R Pseudomonas → translucent, +/- natural pigmentation cream to green
COL-R Acinetobacter → Cream
Other Gram negative COL-R → colourless, natural pigmentation
Other Gram negative COL-S → inhibited
Other gram positive bacteria→ inhibited

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Medium Description

CHROMagarTM COL-APSE is a sensitive and specific medium for the growth of Colistin resistant bacterial pathogens. It is particularly useful as a primary isolation medium in the surveillance and recovery of Colistin resistant bacteria from complex human, veterinary and environmental samples especially those with plasmid mediated MCR-1 or novel mechanisms of polymyxin resistance. 

Gain flexibility using powder rather than ready to use plates: Use the entire pack, or if there is a need for a smaller number of plates, just a portion. If kept under appropriate storage temperature, CHROMagarTM COL-APSE has a 2 years shelf of life. This flexibility is essential to avoid the waste resulting from expired-unused plates. 

Please refer to our IFU and Material Safety data sheet for complete information about the medium. 
CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
All pictures of our products are CHROMagar property and should not be used without our acceptance. 
                                                                                                      Last Update: 19-Jul-2018

Focus on Colistin resistance

Polymyxin E (colistin) and B are increasingly used as antimicrobials in the treatment of multi-drug resistant bacterial infections. Polymyxin resistance, although intrinsic in Gram-positive and some Gram-negative species (Proteus, Morganella, Serratia) is now a problem in a number of other pathogens (Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Salmonella enterica, Klebsiella pneumoniae).
Resistance arises due to mutations / insertions in genes involved in LPS biosynthesis (lpx, pmrA/B, mgrB, phoP/Q) and/ or the acquisition of phosphoethanolomine transferases (PEtN).

Of great concern is the recently described plasmid-encoded PEtN, MCR-1, now found worldwide in a range of animal, human and environmental bacterial isolates.



Antimicrobial Resistance Surveillance of Human Faecal Samples reveals a High Carriage Rate of E. coli MCR-1 in Brunei Darussalam


Muhd Haziq Abdul Momin, Sadia S Miah, David W Wareham Queen Mary Univeristy of London ECCMID Madrid April 2018

A Novel Chromogenic Culture Media (CHROMagar™ COL-APSE) for the Isolation and Differentiation of Colistin Resistant Gram-negative Pathogens


Muhd Haziq F Abdul Momin, David C Bean, Rene S Hendriksen, Marisa Haenni, Lynette M Phee, David W Wareham

CHROMagar COL-APSE: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram- negative pathogens


Abdul Momin et al., Journal of Medical Microbiology DOI 10.1099/jmm.0.000602
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