Clinical Microbiology


CHROMagar™ C.difficile

Click on the picture for
Packaging / Colonies appearance

For detection of Clostridium difficile

Typical Appearance of microrganisms

C. difficile → colourless and fluorescent under UV light at 365 nm
Other bacteria → colourless, no fluorescent or inhibited

Order References 

Please use these references when
contacting your local distributor:
5000 mL........... CD122
To download the certificate of analysis, please indicate your lot number below :


Medium Performance

CHROMagar™ C.difficile is a new fluorogenic culture medium, extremely sensitive and selective, especially designed to simplify and speed up (24 h) the culture of C. difficile.

 1. Rapid detection compared to traditional media: big colonies (around 2 mm) of C. difficile after only 24 h of incubation in anaerobic atmosphere, contrary to traditional media requiring 48 h.

2. High sensitivity and specificity: C. difficile is detected by characteristic fluorescent colonies (under UV light at 365 nm) and the specimen’s flora largely inhibited.

3. Polyvalence: This medium can be used for clinical specimens as well as environmental samples.


Medium Description

CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
All pictures of our products are CHROMagar property and should not be used without our acceptance.      
Last update: 15-Jan-2020

Focus on Clostridium difficile

Clostridium difficile (C. difficile) is the leading cause of nosocomial infectious diarrhea in adults. These infections occur mostly in patients who have both medical care and antibiotic treatment. The symptoms of C. difficile infection are fever, abdominal cramps and severe diarrhea leading to death. In the United States, nearly 250,000 people each year develop C. difficile infections with at least 14 000 deaths (CDC estimate, 2013). Due to the emergence of highly toxigenic C. difficile strains, these infections have become more frequent and more difficult to treat in the last years.

Although PCR has become the leading C. difficile detection technique, culture is essential for strain typing and antimicrobial susceptibility testing. 


Evaluación del CHROMagar C.difficile para el aislamiento de Clostridium difficile en muestras de materia fecal


Berger MA., Faggionato D., Martinez A., Domecq P., Fernández Canigia L. Laboratorio Domecq & Lafage, Hospital Alemán Buenos Aires, Argentina

Implication de Clostridium difficile dans les infections digestives nosocomiales


S. REZGUI et al. - Service de Biologie Clinique - EHS Dr Maouche Mohand Amokrane, Alger

Estudio Comparativo de medios de cultivo (Agar anaerobio BBL, CHROMagar C.difficile, Clostridium difficile Selective Agar (CDSA) BBL) para el aislamiento de C.difficile desde muestras clínicas.


Fabiola Fernandez TM.PhD., Sebastián Baquedano, Annette Trombert Bqca PhD., Victor Silva TM.PhD. - Universidad Mayor, Chile
more publications