Medium Performance
CHROMagar™ C.difficile is a new fluorogenic culture medium, extremely sensitive and selective, especially designed to simplify and speed up (24 h) the culture of
C. difficile.
1. Rapid detection compared to traditional media: big colonies (around 2 mm) of
C. difficile after only 24 h of incubation in anaerobic atmosphere, contrary to traditional media requiring 48 h.
2. High sensitivity and specificity: C. difficile is detected by characteristic fluorescent colonies (under UV light at 365 nm) and the specimen’s flora largely inhibited.
3. Polyvalence: This medium can be used for clinical specimens as well as environmental samples.
Medium Description
CHROMagar™, Rambach™, AquaCHROM™ are trademarks created by Dr. A. Rambach.
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Last update: 15-Jan-2020
Focus on Clostridium difficile
Clostridium difficile (C. difficile) is the leading cause of nosocomial infectious diarrhea in adults.
These infections occur mostly in patients who have both medical care and antibiotic treatment.
The symptoms of C. difficile infection are fever, abdominal cramps and severe diarrhea leading
to death. In the United States, nearly 250,000 people each year develop C. difficile infections
with at least 14 000 deaths (CDC estimate, 2013). Due to the emergence of highly toxigenic C. difficile strains, these infections have become more frequent and more difficult to treat in the
last years.
Although PCR has become the leading C. difficile detection technique, culture is essential
for strain typing and antimicrobial susceptibility testing.
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